Advances in chromatography-based methods for screening active compounds from natural products / 药学学报

Natural products have been a major source of leading compounds in drug discovery. How to effectively screen active compounds from complex matrix remains an interesting topic. In this review, we comprehensively summarized advanced liquid chromatography based approaches in natural products screening, including pre-column, on-column and post-column screening methods. Their advantages, disadvantages and prospect are also discussed.

Effect of auricular-point pressure combined with personalized music therapy on perioperative stress response in primipara / 中国针灸

OBJECTIVE@#To explore the effect of auricula-point pressure combined with personalized music therapy on perioperative stress response in primipara.@*METHODS@#A total of 1000 primiparas who were elective for cesarean section were randomly divided into an observation group and a control group, 500 cases in each one. 1 h before surgery and 3 h, 6 h, 12 h and 24 h after surgery, individualized music relaxation intervention was given for 1 h in the two groups. On the basis of the treatment, auricular-point pressure was added at Pizhixia (AT), Shenmen (TF), Xin (CO), Zigong (uterus), Luanchao (ovary), Neishengzhiqi (TF) in the observation group. Each point was pressed for 1 min each time, repeated once every 15 min, the force is from light to heavy, and it is better to have a feeling of distension, sourness and radiation in the auricle. The scores of anxiety self-rating scale, visual analog scale (VAS), heart rate, systolic blood pressure, thyroid stimulating hormone, cortisol and blood glucose levels were observed in the two groups.@*RESULTS@#A total of 973 primiparas completed the clinical study in the end, including 488 patients in the observation group and 485 patients in the control group. Compared to enrollment, the scores of anxiety self-rating scale before entering the operating room in the two groups were significantly improved (<0.05), and the observation group was superior to the control group (<0.05). Entering the operating room and tracheal extubation, the heart rate and systolic blood pressure of the primiparas in the observation group were more stable than those in the control group, and the differences were statistically significant (<0.05). Entering the operating room, 1 h into the surgery and 3 h after surgery, the thyroid stimulating hormone, cortisol and blood glucose levels of the primiparas in the observation group were significantly better than those in the control group (<0.05). The VAS scores in the observation group at 6 h, 12 h and 24 h after surgery were lower than those in the control group (<0.05).@*CONCLUSION@#Auricular-point pressure combined with personalized music therapy can reduce the perioperative stress response in primipara and promote the improvement of psychological, physiological and emotional.

In vitro study of the secondary mesiobuccal canal of the maxillary second molar / 华西口腔医学杂志

<p><b>OBJECTIVE</b>To get some anatomical knowledge about the second mesiobuccal canal (MB2) of the maxillary second molar of Shandong region by studying the teeth in vitro. The anatomical knowledge may help us to improve the successful rate of root canal therapy of the maxillary molar.</p><p><b>METHODS</b>118 maxillary second molars were collected from the different region in Shandong province. Oblique square of pulp opening form was used, then root canals were detected and got through by C-type file. The rate of MB2 detection and getting through was recorded. By taken X-ray, the numbers, the morphologies and classifications of the root canal systems of these molars were recorded. Then the distance between mesiobuccal canal (MB) and MB2 was observed and measured by endodontic operating microscope and digital measurement. At last, we observed the apical foramen from the surfaces of these teeth, and try to analysis the differences between apical foramen and the end points of the roots.</p><p><b>RESULTS</b>The rate of MB2 detection was 49.15%, among them 82.76% could be got through by files. 108 molars had three roots, among them, for the mesiobuccal root canal system, 46.30% were I root canal system, while II and III systems were 12.96% and 31.48%, respectively. The distance between MB and MB2 was 1.26 mm averaged. The distance between anatomical apical holes and the end points of roots was 1.13 mm averaged.</p><p><b>CONCLUSION</b>MB2 can be detected in most of maxillary second molars in Shandong region. The dentists should try to detect MB2 in the root canal therapy, and should judge work length by clinical behavior and apical locator rather than by X-ray alone.</p>

Screening of effective fraction on experimental gastric ulcer from Pongamia pinnata roots / 中国中药杂志

<p><b>OBJECTIVE</b>To study the effect of extract from Pongamia pinnata roots on experimental gastric ulcer and screen the effective fraction.</p><p><b>METHOD</b>The models of gastric mucosa damage were induced by absolute alcohol in rats and reserpine in mice to observed the effect of ethyl alcohol extract from P. pinnata roots (PRE) and different parts on experimental gastric ulcer.</p><p><b>RESULT</b>PRE, acetic ether extract and n-butanol extract could significantly inhibit the gastric mucosa damage induced by absolute alcohol in rats and reserpine in mice. In absolute alcohol models the gastric ulcer rates of inhibition were 86.4%, 85.4%, 11.5%, respectively. In reserpine models the gastric ulcer rates of inhibition were 37.8%, 33.8%, 19.7%, respectively.</p><p><b>CONCLUSION</b>PRE, acetic ether extract and n-butanol extract could significantly inhibit the gastric mucosa damage induced by absolute alcohol in rats and reserpine in mice. Acetic ether extract from P. pinnata roots has the best effect on experimental gastric ulcer.</p>